Figure 4.

Glutamatergic afferent control of GHRH-GFP neurons. A, Trace recorded in a male GHRH-GFP neuron at −70 mV. B, Cumulated histograms of the mean distributions of the amplitudes (top) and the instantaneous frequencies (bottom) of sEPSCs in male and female GHRH-GFP neurons at −70 mV. *0.0005 < p < 0.05, comparison between genders, one-way ANOVA. C, Typical recording at −50 mV in the presence of TTX 0.5 μm without or with CNQX 20 μm, as indicated by the bold line. D, Same as in B except that the currents were recorded in the presence of 0.5 μm TTX in male (n = 18) and female (n = 12) GHRH-GFP neurons. Calibrations: A, 30 pA, 0.5 s; C, 60 pA, 50 s. ns, Not significant.