Figure 6.

CCh stimulates the presynaptic glutamatergic nerve terminal of GHRH-GFP neurons in males and females. A, Traces successively recorded under control conditions in the presence of TTX 0.5 μm alone and in the presence of TTX plus CCh as indicated (−70 mV, male neuron). Calibration: 30 pA, 5 s. B, C, Cumulated distributions of the amplitudes (top) and the instantaneous frequencies (bottom) of the glutamatergic currents recorded in male (B) and female (C) neurons. Currents were recorded without (bold lines) or with TTX 0.5 μm alone (□), or in combination with CCh 10 μm (◇) or CCh 100 μm (○). For simplification, control distributions are only shown as a line, because the effect of TTX was described above. Differences with TTX levels are indicated in B as *0.01 < p < 0.05 in the 0.15–2.5 Hz range and ^§0.005 < p < 0.05 in the 0.15–80 Hz range, and in C as *0.005 < p < 0.05 in the 0.16–80 Hz range (paired Student's t test). ns, Not significant.