Figure 8.

CCh enhances the afferent GABAergic control of GHRH-GFP neurons in males but not in females. A, Traces were recorded at −30 mV in a male neuron in the absence or presence of CCh. B, C, Cumulated histograms of the mean distributions of instantaneous frequencies of the GABAergic currents in male (B) and female (C) neurons. Currents were recorded in the absence (◇) or presence of CCh at 1 μm (□), 10 μm (◇), or 100 μm (○). D, Traces successively recorded at −30 mV in a male neuron, with or without CCh and TTX (0.5 μm) as indicated. E, Cumulated histograms of the mean distributions of amplitudes (top) and instantaneous frequencies (bottom) of the GABAergic currents recorded in male neurons under control conditions (bold lines; n = 17) in the presence of 0.5 μm TTX alone (□; n = 17), and in combination with 10 μm CCh (◇; n = 17) or 100 μm CCh (○; n = 13). For simplification, control distributions are only shown as a line because the effect of TTX was described in Figure 7. Significance versus control, ^§0.0001 < p < 0.05 in the 1.25–80 Hz range (B, paired Student's t test). ns, Not significant. Calibration: A, 40 pA, 40 s; D, 40 pA, 5 s.