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. 2019 Aug 1;9:11151. doi: 10.1038/s41598-019-47629-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-QuIC analysis showing positive prion seeding activity in MEF_BV cells infected with white-tailed deer and mule deer CWD prions. Cell lysates were collected and processed for RT-QuIC analysis as done for Fig. 5. A positive seeding activity was seen at passage P3 to P9 in cells infected with white-tailed deer (MEF_BV_WTD) prions (a–d) as well as with mule deer (MEF_BV_MD) CWD prions (g–j). (e,k) represent uninfected cell lysate at passage 3 (negative control). (f,l) Represent 1% WTD- and MD-brain homogenate (BH) used as positive control for the assay.