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Reduction in the levels of AChE in the site of microinjection of HypF-N and PI3SH3 aggregates in the NBM. AChE was visualized by histochemistry and DAB staining. Representative photomicrographs (10× magnification) obtained after injection of 1.0 μl aliquots of PBS, 0.01 ng of HypF-N fibrillar aggregates, 5.0 μg of fibrillar, and of 5.0 μg of prefibrillar PI3SH3. The arrows indicate the AChE-positive fibers. For additional details, see Materials and Methods. Scale bar, 60 μm.
