Figure 5.

Total number of neurons in the NBM 7 d after microinjecting HypF-N or PI₃SH3 aggregates. A, Number of neurons in the NBM injected with PBS (top left), monomeric (top right), prefibrillar (bottom right), or fibrillar (bottom left) HypF-N as determined by quantifying the cells stained with an anti-NeuN antibody (10× magnification in each image). ∗∗p < 0.01 versus PBS. B, Microinjection of large amounts of HypF-N or PI₃SH3 prefibrillar aggregates causes more extensive DNA fragmentation than does injection of very low amounts of prefibrillar aggregates of HypF-N or of high amounts of PI₃SH3 fibrillar aggregates as shown by TUNEL staining (40× magnification in each case). Additional details are given in Materials and Methods.