Figure 1.

Depolarization-induced activation of LMO4-mediated transcription and expression pattern of LMO4. A, B, E18 dissociated cortical neurons at 5 DIV immunostained for CAT after being transfected with GAL4-LMO4 and UAS-CAT and either left untreated or stimulated with 50 mm KCl. C, Northern blot of RNA isolated from cortices at different developmental ages hybridized with probes to LMO4 and CLIM2. Then, the same blot was stripped and hybridized with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe for loading control. D–F, Distribution of LMO4 in the developing cortex detected by immunofluorescence with the use of goat anti-LMO4 (green) and mouse anti-MAP2 (red) antibodies on coronal slices of P15 mouse cortex. G–I, Subcellular localization of LMO4 (green) in E18 dissociated cortical cultures counterstained with a nuclear Hoechst dye (red).