Figure 2.

Characterization of LMO4-mediated transcription. E18 dissociated cortical neurons were transfected with the indicated constructs at 3 DIV and treated as indicated at 5 DIV. A, Relative CAT activity in neurons transfected with GAL4-DBD, GAL4-CREB, or GAL4-LMO4 and UAS-CAT and stimulated with 50 mm KCl. B, Relative CAT activity of neurons transfected with GAL4-LHX2 and UAS-CAT and stimulated with 50 mm KCl. C, Relative CAT activity of neurons transfected with GAL4-LMO4 and UAS-CAT and pretreated with pharmacologic inhibitors as indicated, followed by 50 mm KCl stimulation. D, Relative CAT activity of neurons transfected with wild-type GAL4-LMO4 or constructs with cysteine-to-serine point mutations at either cysteine 23 or 87, as indicated, and stimulated with 50 mm KCl. Asterisks indicate significance at p < 0.05. Error bars represent ± SEM.