Figure 3.

Signaling pathways involved in calcium activation of LMO4-mediated transcription. A, Relative CAT activity in E18 dissociated cortical neurons transfected with GAL4-LMO4 and either wild-type or constitutively active forms of CaMK II (CKII), CaMK IV (CKIV), or MEK at 3 DIV and assayed for CAT activity at 5 DIV. B, D, F, Relative CAT activity in E18 dissociated cortical neurons transfected with GAL4-LMO4 at 3 DIV and pretreated with pharmacologic inhibitors, followed by 50 mm KCl stimulation at 5 DIV. C, E, Relative CAT activity in E18 dissociated cortical neurons transfected with GAL4-LMO4 and UAS-CAT, together with indicated constructs, at 3 DIV and stimulated as indicated at 5 DIV. Asterisks indicate significance at p < 0.05. Error bars represent ± SEM.