Figure 6.

Targeted deletion of LMO4 in cortex. A, Cre-recombinase activity in the whole brain of floxed LacZ reporter mice crossed with nex^Cre transgenics. Recombination is seen in the cortex (cx) and hippocampus (hip). la, Lateral amygdaloid nucleus; pir, piriform cortex. B, High magnification of LacZ expression in the cortex after nex^Cre-mediated recombination. C, D, LMO4 expression (green) and MAP2 expression (red) in P15 cortex in control mice (C; n = 3) and LMO4 conditional knock-out mice (D; n = 3) detected by immunofluorescence. Genotypes are indicated in each panel (nc, nex^Cre; f, floxed lmo4). E, F, Immunofluorescence for neuronal (green) and GABAergic (red) markers in cortices of control (E) and LMO4 conditional knock-outs (F). G, Western blot detection of LMO4 protein from isolated cortices of control, lmo4 conditional heterozygous, and lmo4 conditional knock-out animals. H, Genotyping PCR from sample litter of mice bearing various lmo4 alleles (top band, 265 nt floxed allele; bottom band, 180 nt wild-type allele) and nex^Cre alleles (top band, 770 nt wild-type allele; bottom band, 520 nt Cre-recombination allele). The left panel represents PCR results from two lmo4 conditional knock-outs (nc/nc; f/f). The right panel shows PCR results from heterozygous and wild-type animals.