Figure 8.

Segregation of thalamocortical afferents is disrupted in conditional LMO4 null mice. P7 mice cortices were fixed, sectioned, and stained for 5-HT immunohistochemistry. Coronal (A, B) and tangential (C) sections of control mouse (genotype nc/nc;f/+) reveal normal patterning of thalamocortical afferents as revealed by 5-HT immunohistochemistry. Coronal (D, E) and tangential (F) sections of conditional LMO4 null mice (genotype nc/nc;f/f) reveal poor segregation of thalamocortical afferents with small barrels, poorly defined boundaries, and overall decreased cross-sectional area (G; labeled in C and F as rows B, C, D) compared with controls. Asterisks indicate significance at p < 0.05 by paired Student's t test. Error bars represent ± SEM.