Figure 2.

Kindling enhances stimulus-induced JNK phosphorylation. A, C, E, Western blot analysis for Thr283- and Tyr185-phosphorylated JNK (red) and for total JNK (green) was performed on whole-cell lysates collected from the indicated brain regions 15 min after the administration of one electrical stimulus to naive or kindled animals. Representative Western blots are shown, and the p54 and p46 JNK isoforms are indicated. B, D, F, The relative intensity of bands corresponding to phosphorylated p54 and p46 (combined) were determined and this value was divided by that obtained for total JNK. The means of four independent trials ± SEM are plotted. All groups are n ≥ 6. There was a statistically significant increase in the proportion of phosphorylated JNK in the CA1 of kindled animals compared their naive counterparts, and also in animals stimulated for 1 or 2 d (*p < 0.05, paired ANOVA, Student–Newman–Keuls post-tests). Note that increases in phosphorylated JNK were occasionally observed in the CA3 and neocortex (as seen in the Western blot above), but they were not consistent among all animals.