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. 2006 Jun 21;26(25):6863–6872. doi: 10.1523/JNEUROSCI.1322-06.2006

Figure 1.

Figure 1.

A, Transgenic construct used for generation of mutant TαEE and TαEEG38D mice. B, C, Expression of G38D in G38D/Tα−/− and G38D/Tα+/− mice. Immunoblot analysis with anti-rod Tα (K-20) (B) and anti-EE epitope (C) antibodies is shown. EE–Tα*-purified recombinant His6- and EE-tagged Tα* are also shown. The Tα*EE standards were used with the addition of 40 μg of Tα−/− retinal homogenate. Retinas from transgenic G38D/Tα−/−, G38D/Tα+/−, and control (Tα+/+) mice were homogenized and subjected to SDS-PAGE and Western blotting. ret. protein, Retinal protein.