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HPLC analysis of radioactive steroids extracted from frog hypothalamic explants after a 2 h incubation with [3H]Δ5P in control conditions (A) or in the presence of 10−7 m VT (B). The ordinate axis indicates the radioactivity measured in the HPLC eluent. The dashed lines represent the gradient of secondary solvent (percentage solution B). The arrows indicate the elution positions of standard steroids.
