Figure 5.
Effects of CCh on spike responses evoked by DC and CF barrages. A, Representative responses, obtained from a single GN neuron, evoked by suprathreshold stimulus barrages (10 Hz, 1.9 s duration at a rate of 0.1/s) applied at the DC (DC stimulation) and CF (CF stimulation) fibers in controls solution (left) and during superfusion with 10 μm CCh (right). Note the depolarization, the increased summation, and the more sustained response evoked under CCh in both DC- and CF-evoked responses. B, Summary data (n = 6) showing the average number of spikes per stimulus in control ACSF and when 10 μm CCh was added both for DC and CF stimulations. Note the amplified responses evoked by both stimulations under CCh. C, Representative examples of subthreshold stimulus barrages applied at the DC and CF fibers (top and bottom, respectively) in control and CCh. D, Summary data (n = 5) showing the average summation index (Rlast/R1) in control and under CCh for DC- and CF-EPSPs.