Fig. 3.

Histological characterization of α-Syn120 in the striatum and the olfactory bulb. a, Punctate neuropil localization of α-Syn120 in the striatum was consistent with enrichment at synaptic nerve terminals. b, c, TH staining showed enlarged nerve cell processes in striatum of transgenic (b) but not in littermate control (c) mice. h–j, Abnormal axonal morphology was confirmed by phosphorylated neurofilament staining with SMI 31 antibody in transgenic (h, i) compared with littermate control (j) mice. α-Syn120 immunoreactivity (syn-1 antibody) in the olfactory bulb (d) from a transgenic mouse. e, At a higher magnification, TH staining appeared diffusely distributed along the somatodendritic compartment. Abundant α-Syn120-syn-1-positive inclusions (f) were present in the olfactory bulb at all ages examined, showing a different distribution of the protein compared with endogenous full-length α-synuclein in C57BL/6 mice stained with the same antibody (g). Scale bars: a, d, 600 μm; (in h) h–j, 5 μm; (in g) b, c, e, f, 150 μm.