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. 2006 Apr 12;26(15):4054–4062. doi: 10.1523/JNEUROSCI.0603-06.2006

Figure 1.

Figure 1.

HIV-1 Tat expression and its damaging effects on rat embryonic cortical neurons. A, Representative Western blot showing levels of expression of fusion protein EYFP-Tat and control protein EYFP in rat cortical neurons at the indicated time points after nucleofection (see Materials and Methods). EYFP and EYFP-Tat proteins were detected using an antibody that recognizes YFP (top panel). The same blot was probed with anti-Tat antibody (middle panel) and with anti-actin antibody to verify equal loading (bottom panel). B, Expression of EYFP and EYFP-Tat detected by fluorescent light microscopy at 4 and 24 h (hrs) after nucleofection (original magnification, 100×). C, Fluorescent images showing differentiated neurons cotransfected either with pECFP (in blue) and the mitochondrial-targeted dsRed or with pEFP-Tat and dsRed-mito at 32 h after nucleofection (original magnification, 400×). The images were taken using an inverted fluorescent microscope equipped with motorized z-axis and deconvolution software. D, Representative pictures showing expression of EYFP and EYFP-Tat proteins in mature neurons at 4 and 24 h after transfection (original magnification, 200×).