Figure 3.

Pull-down reactions between GST-Tat fusion proteins and protein extracts isolated from differentiated cortical neurons. The Tat mutants were: TatΔ2–36 missing the amino acids 2–35; TatΔ1–48 lacks the first 48 amino acids, and Tat1–56 lacks amino acids 57–86. A, The results of the pull-downs are shown as a Western blot developed with anti MAP2a/b antibody. B, An additional GST-Tat pull-down using a mutant in which the tubulin-binding domain was deleted (Δ36–39). The same blot was probed with anti-MAP2a/b antibody (bottom panel).