Figure 9.

Human tau fails to form pre-tangles in JNPL3 mice on the CHIP^−/− background. Immunohistochemistry with the human tau-specific antibody E1 was used to compare CHT mice (bottom row) with age-matched littermate JNPL3 mice (top row). A, B, E1 immunoreactivity was present in mossy fibers in CA3 of the hippocampus (A, B), but only JNPL3 mice had perikaryal staining in CA3 neurons (A). C, D, In the CA1 sector of the hippocampus, both mice had neuropil staining (C, D), but only JNPL3 had pyramidal neuronal perikaryal staining (C). E, F, Although a few neurons had perikaryal staining in CHIP^−/− mice in the brainstem (F), they were more numerous in the brainstem of JNPL3 mice (E). Neuritic staining was present in the brainstem in both mice.