Figure 2.

Protein synthesis inhibitors administered after contextual reactivation do not affect the stability of mCPP. All values are expressed as mean ± SEM time spent in the drug-conditioned chamber. A, Rats were conditioned to morphine and tested after 1 week (test 1) to reactivate mCPP. Immediately after test 1, one-half of the rats received one injection of cycloheximide or anisomycin (inhibitor; n = 8, of which n = 4 with cycloheximide and n = 4 with anisomycin; black bars), and the other half of vehicle solution (n = 7; white bars). When retested 24 h later (test 2), all rats maintained their mCPP scores. B, Rats were conditioned and tested as described in A. Immediately after test 1, rats received two injections of either cycloheximide (CyX2; n = 8) or vehicle (VeX2; n = 7), 5 h apart. When retested 24 h later (test 2), all rats maintained their mCPP scores. MORP, Morphine; w, week; Ve, vehicle.