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. 2006 Apr 19;26(16):4383–4393. doi: 10.1523/JNEUROSCI.4531-05.2006

Figure 4.

Figure 4.

A, PG production by primary cultures of microglia 30 min after treatment with A23187 in the absence or presence of HQL-79. After incubation in DMEM without FBS for 6 h, the microglia were treated with 5 mm A23187 for 30 min. HQL-79 was added 15 min before A23187 treatment. PG contents in the medium were quantified by enzyme immunoassays. White columns, PGD2; gray columns, PGE2; black columns, PGF2α. n = 3. B, mRNA levels of DP1, DP2, and GFAP before (gray column) and after stimulation of primary cultures of astrocytes with BW245C (black columns) or DK-PGD2 (white columns). Once the astrocytes in the primary culture reached confluence, the cells were rinsed with PBS and subcultured at 5 × 105 cells per well (6-well plate). After a 24 h incubation in DMEM containing 1% FBS, the astrocytes were incubated with BW245C (0.1–100 nm) or DK-PGD2 (0.1–100 nm) for 6 h at 37°C. n = 3. *p < 0.05, **p < 0.01.