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. 2006 Apr 19;26(16):4383–4393. doi: 10.1523/JNEUROSCI.4531-05.2006

Figure 5.

Figure 5.

Alleviated astrogliosis in the mutant twitcher lacking HPGDS or DP1 gene. A–H, GFAP immunostaining in the cerebrum (A–D) and the cerebellum (E–H) of GALC+/+ (A, E), GALCtwi/twi (B, F), HPGDS−/−GALCtwi/twi (C, G), and DP1−/−GALCtwi/twi (D, H) mice. Insets show higher-magnification view of GFAP-immunoreactive astrocytes for each genotype. Scale bars: H, 50 μm; insets, 5 μm. CGL, Cerebellar granular layer; CML, cerebellar molecular layer. I, Quantitative RT-PCR (top) and Northern blot analyses (bottom) for GFAP and G3PDH in GALC+/+, GALCtwi/twi, HPGDS−/−GALCtwi/twi, and DP1−/−GALCtwi/twi. The quantitative PCR analysis of the contents of mRNAs for GFAP and G3PDH was performed by using a LightCycler amplification and detection system. For Northern blotting, total RNA (10 μg) was electrophoresed in an agarose gel, transferred to nylon membranes, and hybridized with 32P-labeled cDNA probes specific for mouse GFAP and G3PDH. n = 3–5. *p < 0.05.