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Representative immunohistochemical images showing the effects of leukocyte-depleting serum (intravenously) on the number of cells expressing β-endorphin (END), EM-1, and EM-2 in inflamed subcutaneous paw tissue. The control group was treated with nonimmune serum (intravenously). All three peptides are localized in granulocytes (1) and mononuclear cells (2). Scale bar, 20 μm.
