Figure 5.

Gel chromatography of spinal cord homogenates from G85R and G127X mice. The hSOD1 in the eluted fractions was analyzed by Western immunoblot, and the resulting relative distributions (percentage) are shown in the figures. The actual immunoblots are shown under the figures. For comparison, the SOD activity of the dimeric mSOD1 is also shown. A, Results for a G85R spinal cord. B, G127X spinal cord. In the immunoblot, both the 17 kDa monomer and a 33 kDa hSOD1 complex of unknown composition are shown.