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. 2006 Apr 19;26(16):4338–4349. doi: 10.1523/JNEUROSCI.3745-05.2006

Figure 2.

Figure 2.

Organization of layer IV neurons but not thalamocortical axon clustering is disrupted in PKARIIβ−/− mice. A, B, Tangential sections from P16 wild-type (A) and PKARIIβ−/− (B) mice stained with CO (n = 6 for both genotypes). Posteromedial whisker barrel patterns (white box in A) are indistinguishable between genotypes. C, D, Tangential sections from P7 wild-type (C) and PKARIIβ−/− (D) mice immunostained with anti-5-HTT antibody (n = 4 for both genotypes), which selectively labels thalamocortical afferents. The barrel pattern is again indistinguishable between genotypes, showing that the gross patterning of thalamocortical afferents in PKARIIβ−/− mice is similar to that of wild types. E, F, Nissl stained tangential sections from P16 wild-type (E) and PKARIIβ−/− (F) mice. In wild-type mice (E), layer IV neurons organize into a barrel wall and hollow pattern characteristic of rodent somatosensory cortex. In PKARIIβ−/− mice (F), only a very rudimentary barrel pattern is visible in posteromedial barrel field. Quantification confirms that the density of neurons in the barrel wall relative to the barrel hollow is significantly higher in wild-type mice than in PKARIIβ−/− littermate mice (1.76 ± 0.05 in n = 4 wild-type mice; 0.93 ± 0.05 in n = 4 PKARIIβ−/− mice; p < 0.001, t test). Scale bars, 500 μm.