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. 2006 Apr 19;26(16):4338–4349. doi: 10.1523/JNEUROSCI.3745-05.2006

Figure 8.

Figure 8.

PKARIIβ−/− mice have a significant reduction in phosphorylation of postsynaptic but not presynaptic PKA targets. A, Western blot examples of synaptosomes prepared from P11 somatosensory cortex of PKARIIβ−/− mice and their littermate controls using antibodies against PKA phosphorylation targets. B, Quantification of normalized ratio of anti-phospho GluR1 (PGluR1, Ser845) to total GluR1 and anti-phospho NR1 (PNR1, Ser 897) to total NR1 reveals a significant difference between PKARIIβ−/− (KO) and wild-type (WT) littermate control mice (*p < 0.005 for both; t test). None of the presynaptic PKA phosphorylation targets such as phospho-synapsin (p = 0.26; t test) and phospho-Rim (p = 0.56; t test) reveal a difference between PKARIIβ−/− mice and their wild-type littermate controls (data not shown). Quantification of the band labeled with an anti-phospho-MAPK (PMAPK) antibody, which recognizes both p44 (top band in A) and p42 (bottom band in A) phosphorylation of MAPK (or ERK), also shows no difference (data not shown; p = 0.51; t test). Error bars indicate SEM.