Figure 4.

Differences in axodendritic distribution of pCaMKII immunolabeling between primary afferent synapses in control dorsal horn and in dorsal horn ipsilateral to capsaicin stimulation. Shown are the axodendritic distributions of pCaMKII immunogold labeling relative to the postsynaptic membrane (presynaptic gold particle distances negated), normalized by the number of synapses, for SP⁺/CGRP⁺ (A), peroxidase⁺ (B), and LTM (C) synapses. The dashed lines indicate the position of the outer surface of the postsynaptic membrane. Error bars indicate SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, two-way ANOVA followed by Bonferroni’s post hoc test. P_caps, Statistical significance of capsaicin effect; P_dist, statistical significance of distance effect; P_i, statistical significance of interaction between capsaicin and distance effects.