Figure 7.
Dependence of presynaptic GABABR activation on temperature and GAD activity. A, Sample traces represent eIPSCs elicited by paired-pulse stimulation in the control and in the presence of CGP55845. Traces represent an average of 40 sequential responses. Data were obtained from the same cell. B, Statistical data showing CGP55845 effects on PPR and release probability at 34°C (n = 7). ∗∗p < 0.01. C, Paired-pulse eIPSCs recorded when GAD was blocked with 3-MP (100 μm, but after >1 h preincubation with 1 mm 3-MP). Traces represent an average of 40 sequential responses. Data were obtained from the same cell. D, Statistical data showing CGP55845 effects on PPR and release probability (n = 6). Error bars indicate SEM. CGP, CGP55845; Cont., control.