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. 2006 Apr 19;26(16):4318–4328. doi: 10.1523/JNEUROSCI.0118-06.2006

Figure 7.

Figure 7.

L-AP-4 reduces colchicine-induced death of TH+ neurons in an MEK-dependent manner. A–D, Midbrain neuronal cultures treated with vehicle (A), colchicine (Col, 10 μm) (B), colchicine and l-AP-4 (100 μm) (C), or colchicine, l-AP-4, and PD98059 (20 μm) (D) were costained for TH (green), TUNEL (red), and NeuN (blue). l-AP-4 markedly reduced the selective toxicity of colchicine on TH+ neurons. The effect was reverted by the MEK inhibitor PD98059. E, F, Midbrain neuronal cultures transfected with HA-tagged wt-MEK1 (E) or dn-MEK1 (F) were treated with colchicine and l-AP-4. Costaining of the cultures for TH (green), HA (red), and TUNEL (blue) showed that the protective effect of l-AP-4 against colchicine toxicity was unaffected by wt-MEK1 (E) and was abolished by dn-MEK1 (F). G, The protective effect of l-AP-4 against colchicine was abolished by the MEK inhibitors PD98059 or U0126 (both at 20 μm), or overexpression of dominant-negative MEK1, but was not significantly affected by overexpression of wild-type MEK1. *p < 0.05 versus Col plus l-AP-4; n = 5. Scale bar: (in A) A–F, 10 μm. Error bars indicate SE.