Figure 6.

SVZ-derived neuroblasts differentiate into mature neurons and form synapses in the striatum by 90 d after MCAO. A, Diagram showing the injection site. B, The pxCANCre plasmid was injected into the lateral ventricle 5 d before MCAO, and the animals were killed 90 d after MCAO. C, Confocal three-dimensional reconstruction image of a GFP/NeuN double-positive cell. At 90 d after MCAO, 29% of the SVZ-derived GFP-positive cells (n = 13) in the striatum expressed NeuN, a specific marker for mature neurons. Scale bar, 20 μm. D, A GFP-positive cell exhibiting neuronal morphology. Scale bar, 20 μm. E, An electron micrograph showing a GFP-positive axon (asterisk) containing presynaptic vesicles. Scale bar, 0.5 μm. F, High-power magnification view of the region indicated by the boxed area in E. The postsynaptic density is indicated by arrowheads.