Figure 6.

Transplanted SKP-derived Schwann cells integrate into white matter and express a myelinating phenotype in the dysmyelinated shiverer cerebellum ex vivo. a–c, Representative 2-week-old cerebellar slice culture as visualized by autofluorescence (a). Immunostaining confirmed that slice cultures from wild-type (b) but not shiverer (c) cultures expressed MBP (red). d, Fluorescence micrograph of differentiated SKPs transplanted into slice cultures and immunostained for MBP (red) after 3 d. The arrowhead indicates transplanted cells. In b–d, nuclei were visualized with Hoechst 33258 (blue). e, f, Fluorescence micrographs of SKP-derived cells on cerebellar slices 2 weeks after transplantation as visualized by immunostaining for MBP (red) and YFP (green) (e) or MBP (red) and S100β (green) (f). Similar results were obtained in three independent slice culture experiments.