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. 2006 Jun 14;26(24):6573–6582. doi: 10.1523/JNEUROSCI.1497-06.2006

Figure 7.

Figure 7.

Comparison of AMPA and NMDA receptor subunit protein expression in control and mGluR7−/− brains. A, Nissl-stained adjacent sections were used to identify the different brain regions after immunostaining. Cyclamen and dark blue dots indicate the sampling sites for striatum (8 circles with a diameter of 0.3 mm) and the perirhinal cortex (4 circles with a diameter of 0.3 mm), respectively. B, Schematic diagram illustrating the sampling method used to compare immunoreactivities in different hippocampal regions (for details, see Materials and Methods). The colors represent layers of the hippocampal formation: red, stratum oriens (SO); green, stratum radiatum (SR); yellow, stratum lacunosum-moleculare (SLM); plum, stratum moleculare of dentate gyrus (SM); blue, hilum of dentate gyrus (H); lilac, stratum lucidum of CA3 (SL); pink, striatum (ST); dark blue, perirhinal cortex (PR). Density readings were taken by placing open circular cursors with a diameter of 0.1 mm at the indicated adjacent positions along SO (8), SR (6), SLM (7), SM (12), H (6), and SL (7). C, D, Representative histoblots of corresponding regions of mGluR7+/+ and mGluR7−/− brains immunostained with antibodies against GluR1–GluR4 (C) or NR1 (D) iGluR subunit proteins. For illustration proposes, grayscale histoblot images were converted to color gradients using gradient mapping. Scale bars, 1 mm. The bar diagrams indicate the pixel density levels (arbitrary units) in various hippocampal and cortical regions in mGluR7+/+ (open bars) and mGluR7−/− (filled bars) animals for the indicated iGluR subunit proteins. Quantitative comparison revealed no significant differences (p > 0.05).