Figure 1.

Measurement of LC neuronal firing rates and MP using the partial access recording mode. A, Top, Sample trace of partial access recording. The MP became stable with the development of partial access compared with the beginning of recording (within 10–15 min). Bottom, Sample trace of on-line firing rate analysis with pClamp (analysis on at ∼4 min after making giga-seal). The firing rate remained stable during the measurement (>60 min), although the amplitude of spikes changed. B, Sample traces in a 20 s window at 15, 40, and 60 min time points from A. MPs were −58.1, −57.6, and −58.3 mV. Note that the firing rate and MP were extremely stable, although the amplitude of spikes progressively increased over time. C, Despite the shifting spike amplitude, the firing rate and MP remained constant throughout the experiment. D, MP measured with the partial access mode is not different from the RMP recorded using the whole-cell mode (n = 16 and 11, respectively; p > 0.5).