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. 2006 Apr 26;26(17):4586–4595. doi: 10.1523/JNEUROSCI.5123-05.2006

Figure 2.

Figure 2.

Movements of postsynaptic scaffolds at inhibitory synapses. Characterization of VeGe synaptic cluster dynamics in cultured spinal cord (10 DIV; A–C) and hippocampal (18 DIV; D–F) neurons is shown. A, VeGe clusters in a spinal cord neuron proximal dendrite. B, Same field as in A with VeGe in green and FM4–64 staining in red. VeGe synaptic and extrasynaptic clusters (arrowheads and arrows, respectively) are identified according to their position relative to FM4–64 puncta. C, Two-frame running subtraction projection (see Materials and Methods for details) corresponding to a time-lapse sequence of the field shown in A and B (101 frames; dt = 1 s). The signal (black) corresponds to fluorescent object displacements (inverted contrast). Note the strong signal associated with large synaptic (arrowheads) and some smaller extrasynaptic (arrow) clusters showing that these objects were moving over short distances around their initial position. D, Visualization of dendritic spines and inhibitory synapses in a hippocampal neuron (18 DIV) cotransfected with mRFP and VeGe. E, F, Two-frame running subtraction projection of mRFP (E) and VeGe (F) time-lapse sequences in the field shown in D (50 frames; dt = 10 s). Note the strong signal associated with dendritic spines (arrows) and VeGe clusters (arrowheads). Displacement of mRFP aggregates in some neurons gives a strong signal in axons (open arrows). Note that some VeGe clusters are less dynamic than others (open arrowheads).