Figure 3.

Velocities of VeGe synaptic clusters. A, Segmentation and individualization of VeGe synaptic clusters (1–4) in the field shown in the inset (arrowheads; VeGe in green, FM4–64 staining in red; same field as in Fig. 2A–C). Individual clusters are identified by a color code. B, Trajectory of the fluorescence “mass center” of the four synaptic clusters highlighted in D. C, Instantaneous velocities (V_inst) as a function of time of four VeGe synaptic clusters in a control cell (same clusters as in A and B) and in a cell exposed to 3 μm latrunculin. Insets, Fluctuations with time of V_inst relative to average value on each trajectory. Note the reduced dynamics of VeGe clusters after latrunculin treatment.