Figure 2.

Effect of growth factors on rotenone-induced SH-SY5Y cell apoptosis. A, SH-SY5Y cells were preincubated with 10 ng/ml bFGF, EGF, or GDNF for 1 h before treatment with 50 nm rotenone for 24 h. B, Dose–response of bFGF protection. Cells were preincubated with 0–10 ng/ml bFGF for 1 h, followed by 50 nm rotenone treatment for 24 h. Data are from two independent experiments of triplicate determinations. At least 6000 cells were counted for each data point. Error bars are SEM. *p < 0.05, ***p < 0.001 (ANOVA) compared with cells treated with rotenone only. V, Vehicle.