Figure 7.

bFGF blocks rotenone-induced AIF nuclear translocation in SH-SY5Y cells. SH-SY5Y cells were pretreated with 10 ng/ml bFGF or vehicle control for 1 h, followed by 0 or 100 nm rotenone for 12 h. Cells were stained with an anti-AIF antibody and Hoechst. Data are deconvolution confocal images and representative of two independent experiments with triplicate determinations. Hoechst staining was pseudocolored to red to better visualize colocalization with AIF. Arrows point to cells with nuclear AIF staining.