Figure 5.

The (Gln-Ala)₃₈ repeat of CA150 influences the nuclear localization of the protein. Eight or 12 weeks after infection of rat striata, the localization of exogenous CA150 and CA150ΔQA was revealed using HA epitope staining. Nuclei were stained using acridine orange. A, Quantification of cytoplasmic and nuclear signals from confocal planes for CA150 compared with CA150ΔQA. Shown are nuclear intensity of HA signals and cytoplasmic area of HA staining. Rats injected with CA150ΔQA alone showed increased cytoplasmic signals and weaker nuclear signals compared with rats injected with CA150 alone, an effect not influenced by htt171-19Q or the time after infection (∗∗∗p < 0.0001). Data are mean ± SD from six fields of view. B, Confocal images of HA epitope staining (red) and nuclear counterstaining (green). The gray arrowheads show nuclear CA150 and white arrowheads show nucleo-cytoplasmic CA150ΔQA.