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Affinity of Leu-Ile and Hsc70 was assayed by QCM. A, Time course of frequency change (−δ F) of dipeptide-immobilized QCM is shown, responding to the addition of Hsc70 to the aqueous solution. B, Binding behavior of Leu-Ile to Hsc70 is dependent on Leu-Ile concentration. C, Frequency change of FK506-immobilized QCM was not observed with Hsc70.
