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. 2006 Mar 22;26(12):3335–3344. doi: 10.1523/JNEUROSCI.5010-05.2006

Figure 5.

Figure 5.

Akt activation induced by Leu-Ile is mediated by Hsp90. A, B, Neurons were stimulated with Leu-Ile (10 μg/ml) alone for 0, 10, 20, and 30 min, or pretreated with GA (10 μm) for 3 h (A) or LY294002 (15 μm) for 2 h (B), followed by Leu-Ile (10 μg/ml) treatment for 30 min. Cell lysates were immunoblotted with antibodies against pAkt and Akt. Each column represents the mean ± SEM (n = 4). Leu-Ile + GA neurons were pretreated with GA followed by Leu-Ile; GA neurons were pretreated with GA alone; Leu-Ile + LY neurons were pretreated with LY294002 followed by Leu-Ile treatment; LY neurons were pretreated with LY294002. **p < 0.01 versus control (0 min); ##p < 0.01 versus Leu-Ile (30 min); $$p < 0.01 versus LY294002. C, Cultures were exposed to 10 μg/ml of Leu-Ile for the periods indicated. Cell extracts were immunoprecipitated (IP) with anti-Hsp90 antibody or control rabbit IgG, followed by immunoblotting (WB). Densitometric data are presented as the mean ± SEM (n = 4). **p < 0.01 versus control (0 min).