Skip to main content
. 2006 Mar 22;26(12):3335–3344. doi: 10.1523/JNEUROSCI.5010-05.2006

Figure 7.

Figure 7.

Leu-Ile increases GDNF expression in a CREB-dependent manner. A, Leu-Ile significantly increased GDNF expression, whereas Pro-Leu and Ile-Pro showed no GDNF-inducing activities. **p < 0.01 versus control (n = 4). B, GDNF mRNA levels induced by Leu-Ile for various periods were studied by real-time RT-PCR. *p < 0.05 and ** p < 0.01 versus control (0 h). C, CREB expression was blocked by CREB antisense ODN, as revealed by Western blotting. D, Neurons were incubated with Leu-Ile (10 μg/ml) for 24 h in the presence of CREB antisense ODN or sense ODN. Data are expressed as a percentage of the control (mean ± SEM; n = 4). **p < 0.01 versus control; *p < 0.05 versus Leu-Ile or Leu-Ile plus CREB sense ODN. E, Neurons were incubated with Leu-Ile (10 μg/ml) for 24 h in the presence of CREB antisense ODN or sense ODN. Data are expressed as a percentage of control (mean ± SEM; n = 4). ***p < 0.001 versus control; ##p < 0.01 versus Leu-Ile or Leu-Ile plus CREB sense ODN. F, Neurons were labeled with anti-GDNF (green) and anti-pCREB antibodies (red). G, CRE-pCREB binding activities were quantified after Leu-Ile treatment for 30 min. **p < 0.01 versus control; ###p < 0.001 verse mutant ODN (n = 4).