Skip to main content
. 2006 Mar 22;26(12):3220–3228. doi: 10.1523/JNEUROSCI.3780-05.2006

Figure 3.

Figure 3.

Glutamate pretreatment induces MAC formation in oligodendrocytes in culture. A, Cells were pretreated with vehicle (top) or glutamate (Glu; bottom; 10 μm, 10 min) and subsequently incubated with rabbit complement (LTC; 30 min). Cells are stained with antibodies to oligodendrocyte-type marker GalC (red; left) and to C5b-9 (green; middle) immunoreactivity. Colocalization of GalC and C5b-9 immunofluorescence is shown in merged images (yellow; right). Pretreatment with glutamate specifically induces MAC formation in oligodendrocyte. Blue fluorescence denotes oligodendrocyte nuclei as revealed by Hoechst 33258. Scale bar, 10 μm. B, Histogram represents the average ± SEM of double-labeled cells from duplicates of three different experiments. *p < 0.0001 compared with vehicle pretreatment plus LTC, non-paired Student's t test. C, Cell death at 3 and 24 h after pretreatment with vehicle or glutamate (10 μm, 10 min) followed by addition of MAC proteins C5b-6, C7, C8, and C9 (0.5 μg/ml each). Values represent average ± SEM. *p < 0.005 compared with vehicle pretreatment followed by C5b-6 plus C7/C8/C9; #p < 0.01 compared with glutamate pretreatment followed by C7/C8/C9.