Figure 3.

Results obtained with various tau constructs expressed together with P60-katanin. Cells in the top row were transfected with the tau constructs alone. Cells in the bottom row were transfected with the tau constructs together with P60-katanin. Four-repeat tau generates microtubule bundling (A) and no indication of microtubule severing (a). The microtubule-binding domain alone generates some bundling, but not as much as the four-repeat isoform (B), and provides some protection against severing (b), but not as much as the four-repeat or the three-repeat (not shown in figure) full-length versions of tau. Two versions of tau that lack the microtubule-binding domain, one naturally occurring (C, c) and one generated by experimental truncation (D, d), show no protection against severing. w/o, Without. Scale bar: (in d) 30 μm.