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. 2006 Apr 5;26(14):3821–3828. doi: 10.1523/JNEUROSCI.5354-05.2006

Figure 4.

Figure 4.

Mapping of the TMD in PS1/SPE-46/7 required for Notch processing. A, PS−/− MEF were transiently cotransfected with APPsw-6myc and the indicated H6X-tagged PS1 constructs. Cell lysates were analyzed for PS expression and processing as in Figure 2A and for full-length APP, APP CTFs, and AICD as in Figure 2C. Secreted Aβ levels were analyzed from the conditioned media as in Figure 2D. B, PS−/− MEF were transiently cotransfected with F-NEXT and the indicated H6X-tagged PS1 constructs. Cell lysates were analyzed for PS expression and processing as in Figure 2A and for processing of F-NEXT by immunoblotting with 9E10 as in Figure 3B. F-Nβ levels were analyzed from the conditioned media as in Figure 3C.