Figure 4.

EMSA with PC12 nuclear extracts. A radiolabeled probe corresponding to the hUcn promoter containing the most proximal half-ERE and medial Sp1 site (halfERE/Sp1 Ucn probe) was used in a gel mobility assay. Ten micrograms of a nuclear extract of PC12 cells transfected with pcDNAERα (A, B, left) or with pcDNAERα-Myc (B, middle and right) was incubated in the different conditions depicted in the figure. C1 and C2 correspond to two retarded DNA/protein complexes. F corresponds to the free probe. Results in A and B are representative of three and two independent experiments, respectively.