Figure 6.

MuSK and agrin expression is increased in rat hippocampus after IA training. A, TaqMan-PCR analysis of MuSK on hippocampal extracts taken from rats trained in IA at 0 h−, 20 h−, and 20 h+. The concentration of mRNA was normalized against that of GAPDH. Data are expressed as mean fold change ± SEM of the ratio 20 h+:0 h− versus 20 h−:0 h− (∗p < 0.05). B, Quantitative Western blot analysis of hippocampal extracts taken from 0 h−, unpaired (unp), and IA trained (20 h+) individual rats. A representative blot per condition is shown. Graphs represent the statistical densitometric analysis of all data. Data were normalized against actin and are expressed as mean percent ± SEM of the 0 h− control mean values (100%). C, Quantitative Northern blot analysis of agrin expressed in hippocampal RNA extracts taken from 0 h−, unpaired, and 20 h+ rats. A representative blot per condition is shown. Graphs represent the statistical densitometric analysis of all data. The same membranes were also hybridized with a cyclophilin probe, which was used for normalization. Data are expressed as mean percentage ± SEM of the 0 h− control mean values (100%). ∗∗p < 0.01; ∗p < 0.05. D, Quantitative Western blot analysis of hippocampal extracts taken from 0 h−, unpaired, and 20 h+ rats. A representative blot per condition is shown. Graphs represent the statistical densitometric analysis of all data. Data were normalized against GAPDH and are expressed as mean percentage ± SEM of the 0 h− control mean values (100%), ∗p < 0.05.