Figure 7.
EGFP and apoE protein and mRNA are present only in injured hippocampal neurons in kainic acid-treated mice. Heterozygous 5-month-old EGFPapoE mice received peritoneal injections of kainic acid (KA; 25 mg/kg) (D–F), and the brains were collected 6 d later. Untreated age-matched heterozygous EGFPapoE mice served as controls (A–C). A, D, Gallyas silver staining of the hippocampal CA1 region. B, E, Merged confocal images of EGFP (green) and anti-apoE (red) in the CA1 region. C, F, In situ hybridization of mouse apoE mRNA in the CA1 region.