Table 2.

DNPH reactivity of complex I subunits immunocaptured from PD brain mitochondria

Band	MW (∼kDa)	PD/CTL	p value (t test)	Peptides found (n)
1	13	1.2	0.02	Not assayed
2	15	1.2	0.08
3	16	1.0
4	18	1.0
5	21	1.1
6	26	1.3	0.04	NDUFB5 (2)
				NDUFB6 (8)
				NDUFB7 (2)
7	35	1.1
8	45	1.0
9	48	1.2	0.03	ND4 (1)
				ND5 (10)
				NDUFS2 (8)
				NDUFV1 (19)
10	76	1.1
11	94	1.6	0.02	NDUFS1 (43)
12	232	1.3	0.02	Not assayed

Complex I macroassemblies were immunocaptured from PD and CTL brain mitochondrial preparations, and then derivatized with DNPH, electrophoresed, and immunoblotted for DNPH as described in Materials and Methods. DNPH(+) bands were quantitated by Quantity One software (Bio-Rad). In a separate gel, bands corresponding to those seen in DNPH blots at ∼26, ∼48, and ∼94 kDa were cut out and analyzed by mass spectrometry. The mass spectrometry output is available as individual Excel files in the supplemental data (available at www.jneurosci.org as supplemental material).