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. 2006 May 10;26(19):5143–5152. doi: 10.1523/JNEUROSCI.0737-06.2006

Figure 5.

Figure 5.

Differential expression of MHC class I molecule in MBP/IFN-γ × PLP/SOCS1 mice. Wild-type (A, B), PLP/SOCS1 (C, D), MBP/IFN-γ (E, F), and MBP/IFN-γ × PLP/SOCS1 (G–J) mouse brains, harvested at postnatal day 21, were dual immunostained with anti-MHC class I/FITC (A, C, E, G, I, green) and anti-flag/Cy3 (B, D, F, H, J, red) antibodies and DAPI nuclear stain (J, blue). Wild-type samples were double negative (A, B). PLP/SOCS1 samples were negative for MHC class I molecule (C) and positive for Flag (D). MBP/IFN-γ samples were single positive for MHC class I molecule (E) and negative for Flag (F). Double-transgenic MBP/IFN-γ × PLP/SOCS1 samples were double positive for MHC class I molecule (G) and Flag (H). Higher magnification of MBP/IFN-γ × PLP/SOCS1 samples (outlined square in G, H) revealed differential distribution of the immunopositivity (I, J); MHC class I-positive cells (large arrows) were negative for Flag, whereas Flag-positive cells (small arrows) were negative for MHC class I molecules. Sagittal sections of corpus callosum. Scale bars: A–H, 20 μm; I, J, 10 μm.