Figure 7.
Disruption of the nodal cytoskeleton does not alter localization of βII spectrin or ankyrinB. A, Cultured hippocampal neurons immunostained for βIV spectrin (red) and βII spectrin (green). βIV spectrin labels the axon initial segment, whereas βII spectrin labels the axon (arrowheads). B, Cultured hippocampal neurons immunostained for pan Nav (red) and βII spectrin (green). Fluorescence intensity profiles in A and B show a reduction in βII immunoreactivity at the axon initial segment. C, D, WT and qv3J optic nerves (C) immunostained for Nav1.6 (green) and βII spectrin (red). Arrowheads show the nodal gap in βII spectrin immunostaining. Scale bars: A, B, 10 μm; C, 3 μm.